Optimization, Characterization and Quantification of Indole Acetic Acid Produced by a Potential Plant Growth Promoting Rhizobacterium Bacillus safensis YKS2 from Yercaud Hills, Eastern Ghats

Plant growth-promoting rhizobacteria (PGPR) have proved to be an effective solution for enhancing growth of various plant species. Five different bacterial isolates extracted from rhizosphere soil were extensively studied the production indole acetic acid (IAA) and among those Bacillus safensis YKS2 strain was found produce substantial quantities IAA. B. characterized submitted National Centre Biotechnology Information (NCBI) (Gen Bank No. MH539636). Optimization IAA with varying pH temperature revealed that maximum at 7 a 37°C. The confirmed quantified by Fourier-transform infrared spectroscopy (FTIR), Thin-layer chromatography (TLC), Gas chromatography-mass spectrometry (GC-MS). PGPR inoculum showed significant (p<0.05) shoot increase (60.00 – 89.00%) root (30.00 90.00%) relative controls in Vigna radiata. This study producing ability can used large-scale promotion.